- Agency for Technical Cooperation and Development
- Narrative Hub
- Health Link South Sudan
- International Organization for Migration
- Danish Refugee Council
- United Nations High Commissioner for Refugees
- Hope Restoration South Sudan
- Office for the Coordination of Humanitarian Affairs
- Youth Empowerment Foundation
- Titi Foundation
- REACH Initiative
- Smile Again Africa Development Organization
- Organization for Peace, Relief and Development
- Care Plus Foundation
- Christian Mission for Development
- Community Action Organization
- Stop Poverty Communal Initiative
- Community Aid for Relief and Development
- Africa Development Aid
- Peace Corps Organization
- Adventist Development and Relief Agency
- Nile Hope
- Touch Africa Development Organization
- HelpEducation South Sudan
- Education Foundation Organization
- Women Aid Vision
- Pilgrims of Hope
- Community in Need Aid
- Oxfam GB
- United Nations Children's Fund
- ACT Alliance / Lutheran World Federation
- Hold the Child Organisation
- International Rescue Committee
- Norwegian Refugee Council
- Plan International
- Centre for Emergency and Development Support
- Polish Humanitarian Action
- Food for the Hungry International
- Community Support Initiative
- Women Advancement Organization
- Support for Peace and Education Development Program
- United Nations Educational, Scientific and Cultural Organization
- Universal Network for Knowledge and Empowerment Agency
- ACT Alliance / Finn Church Aid
- Associazione Volontari per il Servizio Internazionale
- Light for the World
- Charity and Empowerment Foundation
- World Vision International
- Humanitarian Actors for Grassroot Initiative
- Aid Support Community Organization
- Grassroots Relief and Development Agency
- World Relief
- Save a Life International
- Africa Educational Trust
- Windle Trust International
- For Afrika to Thrive
- War Child Holland
- Save the Children
- INTERSOS Humanitarian Aid Organization
- Mercy Corps
- Concern Worldwide
- Salvation and Light Development Action
- Tearfund
- Islamic Relief Worldwide
- Community Advocacy and Relief Organization
- Initiative for Community Prosperity
- Catholic Agency for Overseas Development
- Farmer's Life Development Agency
- Coalition for Humanity South Sudan
- Vétérinaires Sans Frontières - Suisse
- FARM STEW South Sudan
- Child Relief and Support Fund
- Help - Hilfe zur Selbsthilfe e.V.
- Humane Aid for Community Organization
- Organization for Peoples' Empowerment and Needs
- Cordaid International
- African Humanitarian Corps
- Solidarités International
- Rural Community Action for Peace and Development
- ACT Alliance / DanChurchAid
- Green Belt Initiative
- Africa Stand for Children Development Organization
- Deutsche Welthungerhilfe e.V. (German Agro Action)
- Food & Agriculture Organization of the United Nations
- Norwegian People's Aid
- Malteser International Order of Malta World Relief
- ZOA Dorcas South Sudan
- Rii-Yubu Community Development Organization
- Rural Action Against Hunger
- South Sudan Grassroot Initiative for Development
- Samaritan's Purse
- CARE International
- Relief International
- Humanitarian Aid for Development
- National Relief and Development Corps
- Community Aid for Fisheries and Agriculture Development
- Humanity Empowerment and Leadership Promotion Organization
- Catholic Relief Services
- World Food Programme
- Agency for Child Relief Aid
- Action Africa Help International
- MEDAIR
- South Sudan Humanitarian Aid Services
- Christian Recovery and Development Agency
- Nile Initiative Development Organization
- Catholic Medical Missons Board
- John Dau Foundation
- Amref Health Africa
- LiveWell South Sudan
- ONO Aid
- Impact Health Organization
- Mary Help Association
- Episcopal Development Aid
- Médicos del Mundo Spain
- GOAL
- The Rescue Initiative South Sudan
- Children Aid South Sudan
- United Nations Population Fund
- The Mentor Initiative
- Christian Mission Aid
- Medici con l'Africa CUAMM
- Handicap International - Humanity & Inclusion
- HealthNet TPO
- Community Initiative for Development Organization
- Malaria Consortium
- United Networks for Health
- Universal Intervention and Development Organization
- World Health Organization
- Action Against Hunger
- Southern Sudan Healthcare Organization
- Medicair - South Sudan
- Sudan Medical Care
- Rural Health Services
- International Medical Corps UK
- Healthcare Foundation Organization
- South Sudan Health Association
- Health Action Africa (South Sudan)
- Alliance For Action Aid
- Interchurch Medical Assistance World Health
- Volunteer Organization for the International Co-operation la Nostra Famiglia
- Yo' Care South Sudan
- Johanniter International
- Humanitarian Action to Restore and Empower Communities for Development
- Universal Network for Child Defence Rights
- American Refugee Committee (Alight)
- Community Resilience and Emergency Support Aid
- Andre Foods South Sudan
- Charity Mission Corps
- Grassroot Empowerment and Development Organization
- Sudan Evangelical Mission
- Lulu Care - South Sudan
- Greater Upper Nile Organization
- ACT Alliance / Norwegian Church Aid
- The Israel Forum for International Humanitarian Aid
- Mobile Theatre Team
- Girl Child Africa Foundation
- Active Youth Agency
- Confident Children out of Conflict
- Voice of the Peace
- Nonviolent Peaceforce
- Dialogue and Research Institute
- Mobile Humanitarian Agency
- Relief Agency
- The Organisation for Children's Harmony
- Support the Empowerment of Women and their Rights for Development
- Operation Save Innocent Lives - Partners in Relief and Development
- United Nations Mine Action Service
- Institute for Promotion of Civil Society
- Reach Africa Organization
- Trust Guarantee Community Development Aid
- Humanitarian and Development Consortium
- Care for Children and Old Age in South Sudan
- Youth Empowerment and Development Aid
- Child Hope Organization
- Food Agriculture and Disaster Management
- International Aid Services
- Peace Winds Japan
- Rural Water and Sanitation Support Agency
- The Rescue Mission
- Nile Sustainable Development Organization
- Relief Corps Organization
- Water for South Sudan
- Alaska Sudan Medical Project