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- Samaritan's Purse
- Action Africa Help International
- United Nations Children's Fund
- Concern Worldwide
- MEDAIR
- Community Action Organization
- South Sudan Humanitarian Aid Services
- Christian Recovery and Development Agency
- Nile Initiative Development Organization
- International Organization for Migration
- Catholic Medical Missons Board
- John Dau Foundation
- Amref Health Africa
- LiveWell South Sudan
- ONO Aid
- Impact Health Organization
- Mary Help Association
- Episcopal Development Aid
- Save the Children
- Médicos del Mundo Spain
- GOAL
- The Rescue Initiative South Sudan
- Children Aid South Sudan
- United Nations Population Fund
- The Mentor Initiative
- Women Aid Vision
- Christian Mission Aid
- Medici con l'Africa CUAMM
- Nile Hope
- Touch Africa Development Organization
- Coalition for Humanity South Sudan
- Hold the Child Organisation
- World Vision International
- Islamic Relief Worldwide
- Handicap International - Humanity & Inclusion
- HealthNet TPO
- Community Initiative for Development Organization
- International Rescue Committee
- World Relief
- Relief International
- CARE International
- Malaria Consortium
- Health Link South Sudan
- United Networks for Health
- Community in Need Aid
- Universal Intervention and Development Organization
- World Health Organization
- Universal Network for Knowledge and Empowerment Agency
- For Afrika to Thrive
- Action Against Hunger
- Cordaid International
- Southern Sudan Healthcare Organization
- Medicair - South Sudan
- Sudan Medical Care
- Rural Health Services
- International Medical Corps UK
- Healthcare Foundation Organization
- South Sudan Health Association
- Organization for Peoples' Empowerment and Needs
- Christian Mission for Development
- Health Action Africa (South Sudan)
- Alliance For Action Aid
- Catholic Relief Services
- Malteser International Order of Malta World Relief
- Interchurch Medical Assistance World Health
- Volunteer Organization for the International Co-operation la Nostra Famiglia
- Yo' Care South Sudan
- Child Relief and Support Fund
- Johanniter International
- Humanitarian Action to Restore and Empower Communities for Development