- Hope Restoration South Sudan
- United Nations High Commissioner for Refugees
- Danish Refugee Council
- International Organization for Migration
- Health Link South Sudan
- Narrative Hub
- Agency for Technical Cooperation and Development
- Titi Foundation
- Youth Empowerment Foundation
- REACH Initiative
- Office for the Coordination of Humanitarian Affairs
- World Vision International
- Charity and Empowerment Foundation
- Light for the World
- Associazione Volontari per il Servizio Internazionale
- ACT Alliance / Finn Church Aid
- Universal Network for Knowledge and Empowerment Agency
- United Nations Educational, Scientific and Cultural Organization
- Support for Peace and Education Development Program
- Women Advancement Organization
- Community Support Initiative
- Food for the Hungry International
- Polish Humanitarian Action
- Centre for Emergency and Development Support
- Justice for Children Organization (South Sudan)
- Universal Intervention and Development Organization
- Plan International
- Norwegian Refugee Council
- Africa Educational Trust
- United Nations Children's Fund
- World Relief
- Grassroots Relief and Development Agency
- International Rescue Committee
- Hold the Child Organisation
- Save the Children
- ACT Alliance / Lutheran World Federation
- Oxfam GB
- Community in Need Aid
- Pilgrims of Hope
- Women Aid Vision
- Education Foundation Organization
- HelpEducation South Sudan
- Touch Africa Development Organization
- Nile Hope
- Adventist Development and Relief Agency
- Peace Corps Organization
- Humanitarian Aid for Change and Transformation
- Africa Development Aid
- Save a Life International
- Community Aid for Relief and Development
- Stop Poverty Communal Initiative
- Community Action Organization
- Christian Mission for Development
- Care Plus Foundation
- Smile Again Africa Development Organization
- Organization for Peace, Relief and Development
- Community Empowerment Network
- Humanitarian Actors for Grassroot Initiative
- Mercy Corps
- INTERSOS Humanitarian Aid Organization
- Africa Stand for Children Development Organization
- Samaritan's Purse
- Green Belt Initiative
- ACT Alliance / DanChurchAid
- Rural Community Action for Peace and Development
- Agency for Child Relief Aid
- South Sudan Grassroot Initiative for Development
- For Afrika to Thrive
- Solidarités International
- Community Aid for Fisheries and Agriculture Development
- African Humanitarian Corps
- Cordaid International
- Medicair - South Sudan
- Organization for Peoples' Empowerment and Needs
- Humane Aid for Community Organization
- Deutsche Welthungerhilfe e.V. (German Agro Action)
- Rural Women for Development South Sudan
- Help - Hilfe zur Selbsthilfe e.V.
- Child Relief and Support Fund
- FARM STEW South Sudan
- Relief International
- Vétérinaires Sans Frontières - Suisse
- Community Development Aid
- Coalition for Humanity South Sudan
- Food & Agriculture Organization of the United Nations
- CARE International
- Farmer's Life Development Agency
- Handicap International - Humanity & Inclusion
- Catholic Agency for Overseas Development
- Community Initiative for Development Organization
- Help Restore Youth South Sudan
- Initiative for Community Prosperity
- Community Advocacy and Relief Organization
- Islamic Relief Worldwide
- Action Against Hunger
- Tearfund
- Christian Recovery and Development Agency
- Salvation and Light Development Action
- Norwegian People's Aid
- World Food Programme
- Relief Corps Organization
- South Sudan Development Agency
- African Leadership Skills Initiative
- Community Organization for Emergency and Rehabilitation
- Concern Worldwide
- Food Agriculture and Disaster Management
- Community Initiative for Sustainable Development Agency
- South Sudan Health Association
- Healthcare Foundation Organization
- International Medical Corps UK
- Rural Health Services
- Sudan Medical Care
- Health Action Africa (South Sudan)
- Southern Sudan Healthcare Organization
- Alliance For Action Aid
- World Health Organization
- United Networks for Health
- Malaria Consortium
- HealthNet TPO
- Medici con l'Africa CUAMM
- Christian Mission Aid
- The Mentor Initiative
- United Nations Population Fund
- Children Aid South Sudan
- The Rescue Initiative South Sudan
- GOAL
- Médicos del Mundo Spain
- Episcopal Development Aid
- Mary Help Association
- Impact Health Organization
- ONO Aid
- LiveWell South Sudan
- Amref Health Africa
- John Dau Foundation
- Catholic Medical Missons Board
- Nile Initiative Development Organization
- South Sudan Humanitarian Aid Services
- MEDAIR
- Action Africa Help International
- Grassroot Empowerment and Development Organization
- Charity Mission Corps
- Andre Foods South Sudan
- Community Resilience and Emergency Support Aid
- American Refugee Committee (Alight)
- National Relief and Development Corps
- Universal Network for Child Defence Rights
- Relief Agency
- Mobile Humanitarian Agency
- Dialogue and Research Institute
- Nonviolent Peaceforce
- Voice of the Peace
- Confident Children out of Conflict
- Active Youth Agency
- Support the Empowerment of Women and their Rights for Development
- Girl Child Africa Foundation
- Institute for Promotion of Civil Society
- War Child Holland
- Mobile Theatre Team
- The Israel Forum for International Humanitarian Aid
- Operation Save Innocent Lives - Partners in Relief and Development
- United Nations Mine Action Service
- ACT Alliance / Norwegian Church Aid
- Mines Advisory Group
- Greater Upper Nile Organization
- Lulu Care - South Sudan
- Sudan Evangelical Mission
- Youth Empowerment and Development Aid
- Care for Children and Old Age in South Sudan
- Trust Guarantee Community Development Aid
- Reach Africa Organization
- Humanitarian and Development Consortium
- Water for South Sudan
- Aid Support Community Organization
- Nile Sustainable Development Organization
- The Rescue Mission
- Rural Water and Sanitation Support Agency
- Upper Nile Youth Mobilization for Peace and Development Agency
- Peace Winds Japan
- International Aid Services
- Humanitarian Action to Restore and Empower Communities for Development
- Dorcas Aid International
- Invisible Women Inclusion Network
- Child Hope Organization